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:: Volume 8, Issue 2 (صفحات انگلیسی از 7 تا 12(چکیده) 2009) ::
Materials & Energy 2009, 8(2): 133-142 Back to browse issues page
Investigation of Morphological Identification in Pinus eldarica Medw. and its two Natural Generated Types Using Electrophoretic Pattern of SDS-PAGE, Morphological Traits and Some Physiological Parameters
Abstract:   (1742 Views)
In order to consideration and distinguishing of Mondell pine species (Pinus eldarica) and its two natural generated morphotypes, needles samples were collected from three years old seedlings in similar conditions and then extracted. Protein band patterns of the samples were studied and compared using electrophoresis method on polyacrylamide gel in the SDS-PAGE system. 12 morphological traits related to seedlings and mature individuals of the three pines, compared with each other. The amount of total protein also obtained using Bradford procedure and content of chlorophyll a and b measured by spectrophotometer with Harborne method. One dimensional electrophoresis of proteins showed perfectly similar isozyme patterns for each type (i.e. Mondell pine, Ball-shaped and Conical-shaped pines). Furthermore the amount of total protein and chlorophyll a and b contents of samples did not showed significant difference using ANOVA test. But 11 features of morphological measured traits were more in Mondell pine than the new generated pines. These results indicate the nonexistence of biochemical differences among these pines in spite of obvious structural and morphological differences.
Keywords: Mondell pine, Total protein, Electrophoresis, SDS-PAGE, Morphotype
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Investigation of Morphological Identification in Pinus eldarica Medw. and its two Natural Generated Types Using Electrophoretic Pattern of SDS-PAGE, Morphological Traits and Some Physiological Parameters. Materials & Energy. 2009; 8 (2) :133-142
URL: http://jsci.khu.ac.ir/article-1-1229-en.html


Volume 8, Issue 2 (صفحات انگلیسی از 7 تا 12(چکیده) 2009) Back to browse issues page
نشریه علوم دانشگاه خوارزمی Quarterly Journal of Science  Kharazmi University